Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia.

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Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia.

Sister chromatid exchanges, which may reflect chromosome repair in response to certain types of DNA damage, provide a means of investigating the increased chromosome fragility characteristic of Fanconi's anemia. By a recently developed technique using 33258 Hoechst and 5-bromodeoxyuridine, it was observed that the baseline frequency of sister chromatid exchanges in phytohemagglutinin-stimulated...

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Induction of sister chromatid exchanges in human lymphocytes by DTPA.

The chelating agent, diethylenetriaminepentaacetic acid (DTPA) is a non-registered drug used in the treatment of over-exposure to heavy metals such as iron, zinc and lead (Fahey et al., 1961; Brugsch et al., 1965). DTPA has also been used on a few occasions in the United Kingdom when individuals have inhaled plutonium dust or received contaminated wounds (Schofield and Dolphin, 1974). These pat...

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Cytotoxicity and induction of sister chromatid exchanges in human and rodent brain tumor cells treated with alkylating chemotherapeutic agents.

Rodent (9L and 9L-2) and human (SF-126 and SF-188) brain tumor cells were treated with methylating, ethylating, and chloroethylating nitrosoureas. 9L-2 and SF-188 cells were 10-fold and 5.4-fold more resistant to the cytotoxic effects of 1-(2-chloroethyl)-1-nitrosourea (CNU) than were 9L and SF-126 cells. SF-188 cells were 2.5- to 3-fold more resistant to N-methyl-N-nitrosourea (MNU) and strept...

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Analysis of sister-chromatid exchanges and tumorigenicity in cell hybrids.

The relationship between the 'spontaneous' frequency of sister-chromatid exchanges (SCE) and tumorigenicity was studied in a series of hybrids between a C57BL melanoma cell line and diploid cells, but no correlation was found between the 2 variables. Hybrids in which malignancy was suppressed and malignant segregants derived from them showed virtually identical SCE frequencies. Variation of SCE...

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Solution Radioactivated by Hadron Radiation Can Increase Sister Chromatid Exchanges.

When energetic particles irradiate matter, it becomes activated by nuclear reactions. Radioactivation induced cellular effects are not clearly understood, but it could be a part of bystander effects. This investigation is aimed at understanding the biological effects from radioactivation in solution induced by hadron radiation. Water or phosphate buffered saline was activated by being exposed t...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1975

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.72.10.4066